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After the initial phase, your treatment will depend on whether your vitamin B12 shortage is caused by your food or whether it is resulting in any neurological issues, such as issues with thinking, memory, and behaviour, Dr Aravinda commented

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Samples were withdrawn after 0, 2, 4, 24, 48 and 72 hours and incubated with enzyme and detection reagent for 10 minutes before detection using a SpectraMax i3 plate reader at 550 nm excitation and 595 nm emission