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The debilitating, slow damage created by high levels of blood sugar (glucose) destroys everything from your blood vessels to your eyes, kidneys, and nerves over time due to oxidative stress (remember the free radicals or reactive oxygen species (ROS)?)

For the next measurement some pellets are immersed in a buffer (which acts as an artificial cytosol) containing ADP at pH = (5.5 0.2) which biases the TL towards scavenging ADP to be converted to ATP in the mitochondria

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doi: 10.1038/cdd.2014.150 23 GhezellouP.von BlowV.LuhD.BadinE.AlbuquerqueW.RoderfeldM.et al

Samples were filtered through cell strainer tubes and analysed by a FACS Canto II system equipped with 405-nm, 448-nm and 633-nm laser lines