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This vulnerability arises from multiple converging factors comprising increased oxidative stress, disrupted iron homeostasis, depleted antioxidant reserves, and altered lipid composition (Park et al., 2021

The AIMS (Amplification of Inter-Methylated Sites) method uses a combination of the methylation-sensitive SmaI and its isoschizomer methylation-insensitive PspAI enzyme to construct fragments to which adaptors are ligated to perform a further whole-genome PCR reaction [94]

The first and rate-limiting step of glutathione synthesis, the formation of -glutamylcysteine from glutamate and cysteine, is normally subject to negative feedback inhibition by glutathione itself [41]

In our study, as the intracellular -GC level was only increased slightly compared with that of original strain W303-1b when F pathway was introduced, we artificially introduced a flexible six-glycine linker between the two enzymes of G pathway as well as P pathway to improve the coupling efficiency by mimicking the native domain fusion of GshF

Ilhan ZE, Brochard V, Lapaque N, Auvin S, Lepage P