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Factors Influencing Dosage and Frequency Several factors can influence the dosage and frequency of Vitamin B12 injections: Severity of Deficiency: More severe deficiencies require higher doses and more frequent injections initially

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The K m for this, and other substrates, may be determined experimentally by measuring the enzyme activity at different substrate concentrations and curve fitting the data using Kaleidagraph V

FMOCderivatization products were analyzed by loading samples onto an Agilent Zorbax Eclipse XDB-C18 column (50 4.6 mm 1.8 m) with mobile phase A (0.05% formic acid in milliQ water) and mobile phase B (acetonitrile)