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Cell lysis and protein extraction For protein extraction, cells were disrupted with a cell scraper in a lysis buffer consisting of 50 mM HEPES-Na, pH 7.2, 0.5 M NaCl, 0.5% (v/v) NP-40, 0.05% LDS and 1Pierce EDTA-free protease inhibitor cocktail (Thermo, Australia) in Milli-Q H 2 O and then vortexed

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Jomov K, Hudecova L, Lauro P, Simunkova M, Alwasel SH, Alhazza IM, Valko M (2019) A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3,4-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study

Transcription in the PURE CFE system has been monitored without modifying the transcript using Frster resonance energy transfer (FRET) based binary DNA primer probes