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Athletes with increased metabolic demands and iron loss through sweat and training

doi: 10.1002/acr.21660 42 ParksCGHoppinJADe RoosAJCostenbaderKHAlavanjaMCSandlerDP

Caspase 3 activity assay Fifty milligrams of frozen remnants livers were lysed in caspase 3 activity buffer (20 mM PIPES, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS, 10% sucrose, pH 7.2)

Indeed, HA-tagged NEDD8 or ubiquitin, but not SUMO2 or SUMO3, immunoprecipitated modified high-molecular weight IL-1 when expressed in 293T cells (Supplementary Fig