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Interestingly, in Phd2 deficient neurons showing a comparatively less pronounced increase of HIF-1 protein abundance (data not shown), PFKFB3 protein levels remained stable, while transcript levels were slightly but significantly increased (Figure S6B) suggesting that up-regulation of PFKFB3 expression, which exceeds the constant proteasomal degradation of PFKFB3 in neurons, may require strong activation of HIF-1