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After sequential washes with low salt buffer (20 mM, Tris pH 8.0, 2 mM EDTA, 150 mM NaCl, 1% Triton, 0.1% SDS), high salt buffer (20 mM Tris pH 8.0, 2 mM EDTA, 500 mM NaCl, 1% Triton, 0.1% SDS), LiCl buffer (10 mM Tris pH 8.1, 0.25 M LiCl, 1 mM EDTA, 1% NP40, 1% IGEPAL) and TE buffer, the DNA-bound immunocomplexes were eluted and the DNA were extracted by PCR clean up kit

Melanin is produced by cells called melanocytes, and the amount and type of melanin determine our skin color

Foerster EG, Mukherjee T, Cabral-Fernandes L, Rocha JDB, Girardin SE, Philpott DJ

10.18433/j3nc79 122 PivinaL.SemenovaY.DoaM