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Description

Drift tubemass spectrometer for studies of lowenergy ionmolecule reactions

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For the purification of GST-GSK3, harvested cells were resuspended in lysis buffer (20 mM Tris pH 7.4, 300 mM NaCl, 5% glycerol, 0.01% Triton X-100, 5 mg/mL DNase I, 1 mM DTT and EDTA-free protease inhibitor cocktail