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One thing is already clear to the team they will not be simply using serum B12 levels to assess the success of oral treatment

Low resolving triple quadrupole systems (here a quadrupole-linear ion trap hybrid) generally reach lower limits of detection based on a higher duty cycle in optimized MRM modes

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Appl Biol Chem 68(2):123145

After 72 h, conditioned medium was harvested and subjected to batch IMAC using nickel nitrilotriacetic acid resin (Invitrogen) with peptidase inhibitors (Roche cOmplete), followed by SEC on a Superdex 75 Increase 10/300 GL column