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The frequency and dosage vary depending on the individual's needs and goals

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In particular, the use of incell NMR spectroscopy in human cells possesses great potential as a means of dissecting pathways of protein maturation and posttranslational modification under nearphysiological conditions of pH, redox potential, viscosity and the presence of all relevant interaction partners

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After 30 min at 4 C, cells were washed in perm/wash buffer and resuspended with secondary Ab anti-Rabbit IgG FITC (Fisher Scientific 15303926) or PE (CST 79408 S)