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Addressing this instability remains a major challenge in synthetic biology, and it is an area where AI-assisted design is expected to have the greatest impact ( Although some engineered bacteria utilizing genomic integration have entered clinical trials, Cubillos-Ruiz et al

Multiturnover ubiquitylation assay Fluorescent-FKBP12 (0.5 M), CUL1NEDD8-RBX1 (0.5 M), FBXO22-SKP1 (0.5 M), ARIH1 (0.4 M), UBE2L3 (2.0 M) and UBE2R2 (2.0 M) were mixed with buffer (50 mM HEPES, 100 mM NaCl, 7.5 mM MgCl 2 , 5 mM ATP, 0.5 mg ml 1 BSA, pH 7.5) and either DMSO or compounds (10 M)

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