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Because native hGH residue 176 is phenylalanine, AOD9604 and the unmodified 176-191 fragment are both 16-residue peptides that differ only at the first position

Kinetic studies also confirmed that SIRT4 was the first mammalian sirtuin discovered to be physiologically regulated by the [NAD + ]/[NADH] instead of by [NAD + ] (Pannek et al., 2017) as 30 M NADH, a physiological concentration found in the mitochondrial matrix (Sallin et al., 2018), inhibited SIRT4 activity