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Store lyophilized material at 4 F (20 C) or below Allow vial to reach room temperature before opening Protect from light, heat, and humidity Use sterile laboratory equipment during preparation Avoid repeated freezethaw cycles Label reconstituted samples with preparation date and concentration Following these practices helps support consistent and reproducible laboratory results

Smaller and more targeted than the proteins that build tissue

Typical daily range: 200300 mcg once daily across a 48 week course

Dose-ranging studies evaluated 1mg, 4mg, and 8mg daily for 28 days in patients with sarcoidosis-related neuropathy

Nevertheless, use of metformin may decrease vitamin B 12 status: a meta-analysis of four short-term intervention trials (up to 4 months duration) in patients with type 2 diabetes found that metformin use decreased serum cobalamin concentrations by 57 pmol/L, which could have clinical implications if such patients have suboptimal vitamin B 12 status (146)