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Images were collected using a CFI Plan Apochromat Lambda D oil immersion objective (60, NA = 1.4) and for roGFP imaging a CFI Plan Apo VC water immersion objective (60, NA = 1.2)

The significance of differences in protein abundance was determined with a Paired T-test for sibling cohort comparisons and a T-test for comparisons of unrelated groups such as Stage 1 versus Stage 3

A key feature is its flexible loop 2, which may serve a gating function for substrate binding specificity

doi: 10.1691/ph.2019.8674 128 WangJNiZDuanZWangGLiF