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Additionally aldehyde toxicity may occur due to PLP concentration along, with the formation of reactive intermediates and competitive inhibition of PLP dependent enzymes

PMID: 8118430 Collins S, Surwit RS

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After centrifugation at 14,000 g at 4 C for 2 min, the supernatants were evaporated to dryness in a SpeedVac concentrator at 45 C for 90 min at 1.5 atm pressure