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As LPS enhanced the phosphorylated Ser42 and Tyr198 in GSTP, we transfected GSTP mutants including GSTP-S42A, GSTP-S42D, GSTP-Y198F, GSTP-Y198D, and GSTP-S184D into RAW264.7 cells and then treated cells with LPS for 18 h

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Inflammatory cytokines including TNF- and interleukin (IL)-1 & Scott et al, 2010) (Figures 5 & 6)

Expecting visible changes in the first 1-2 weeks sets unrealistic expectations

It has been observed that Gpr50 is a target of SIRT1 [72] and treatment of HEK-293 cells with resveratrol (50 M) for 48 h increased SIRT1 and GPR50 gene expressions, as well as SIRT1 protein expression