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They should have no pre-existing health conditions, as they could interfere with the healing process after the surgery

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The remaining 23 mL of each culture was quickly filtered under vacuum (Air Cadet vacuum/pressure station, Thermo), immediately washed with cold saline solution (9.5 g L 1 NaCl at 12 C) and quenched in cold methanol/water (1:1 v/v) at 30 C as described by Smart et al

Glycine in Skincare and Anti-Ageing As collagen's primary structural component, glycine plays an important role in maintaining skin elasticity and youthfulness