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The quantification of GSH was carried out by comparing the fluorescence produced by the samples versus that of a standard solutions curve with known concentrations of GSH.11 To measure GSSG concentration, 25L aliquots of saliva were incubated with 10L of a N-ethylmaleimide solution (NEM, 5mg/mL in distilled water) for 40min at room temperature

Sourris KC, Harcourt BE, Forbes JM (2009) A new perspective on therapeutic inhibition of advanced glycation in diabetic microvascular complications: common downstream endpoints achieved through disparate therapeutic approaches

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Being an essential signal molecules of human body, SCFAs are also involved in the regulation of metabolism by gut microbiota, such as cholesterol biosynthesis, glucose homeostasis and insulin sensitivity (Lee et al., 2020)

There is extensive clinical evidence that systemic oxidative stress contributes to glaucomatous optic neuropathy