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The second isocratic step of the elution gradient was purposely extended far beyond the elution of vicine and convicine (i.e., until 14 min), since this enabled an improvement of the separation between them and other polar compounds contained in the flour extract, whose peaks were clearly detected in the LC-ESI-MS Total Ion Current (TIC) chromatogram obtained for the flour extract, also shown in Fig

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74 Ethanol tampers with the function of multiple enzymes, including MAT, BHMT, and various MTs