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Importantly, folic acid should not be given alone when B12 deficiency is possible, as this may worsen neurological symptoms

Masharani UB, Becker J

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[DOI] [PubMed] [Google Scholar] 50.Sharififar F., Mirtajadini M., Azampour M.J., Zamani E

Then, cells were incubated with 5 M of MitoSOX TM Red dye in PBS with 0.5% serum at 37 C with 5% CO 2 for 30 min before being assessed in a cytoFLEX cytometer (Beckman Coulter)