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To determine whether these decreases in albumin may contribute to progression of ALD, we subjected ALB KO and ALB Het mice to the 10d + binge model of ethanol consumption (NIAAA model) and found that ALB KO mice exhibited worse liver injury than ALB Het mice

Materials and methods Chemicals and reagents We acquired thiobarbituric acid (TBA, T5500), epigallocatechin 3 gallate (EGCG, PHL89656), astaxanthin (Ast, SML0982), curcumin (C1386), trichloroacetic acid (TCA, T6399), D galactose (D-gal, G0750) from Sigma-Aldrich (Germany), and protease inhibitor cocktail from Sigma, Saint Louis, MO, USA

The substrates and oxidases utilized by these compartments vary, leading to differences in reaction rates and intensities

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