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Adenosine-5-phosphosulfate kinase is essential for Arabidopsis viability

For 96-well plates, 10 L of prepared reagent (1:50 ratio of monochlorobimane (MCB) to Tris assay buffer, per manufacturers instructions) was added to wells containing 100 L of culture media and incubated for 30 min at 37 C, washed with PBS, and imaged using an Olympus IX70 microscope with a DAPI filter (Excitation/Emission: 358 nm/461 nm)

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A chemical mechanism for the inhibition of SARS-CoV-2 Mpro cysteine-145 by ebselen and its derivatives has been published (Reference Amporndanai, Meng and Shang81)

Pharmaceutics 13:1270