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2.2.2 Reagent preparation Prepare 100 mM NEM stock solution: Add fresh NEM powder to 10 mM Ammonium formate solution (pH 7)

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They carry proteins, lipids, growth factors, and genetic material to stimulate cellular repair, regeneration, and collagen production

In brief, sections were washed with distilled water before being immersed in 1 Black-Gold II solution and incubated at 65 C for 14 min, until staining of the myelinated regions was becoming apparent

Additionally, DSIP demonstrates antioxidant properties and helps normalize various neuroendocrine functions, contributing to its comprehensive effects on sleep quality and stress management