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Figure 3 Additionally, MB demonstrated a moderate citotoxicity profile against hepatocarcinoma cell line HepG2, yielding an EC 50 of 2.68 1.81 M ( Supplementary Material Figure 1 )

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Preclinical studies reveal that VEGF-mediated vascular abnormalities not only impair drug delivery but also establish an immunosuppressive niche through multiple pathways: (1) upregulation of PD-L1/CTLA-4 expression on endothelial and immune cells (80, 81), (2) recruitment of regulatory T cells and myeloid-derived suppressor cells (32), and (3) induction of hypoxia-driven adenosine accumulation (82)

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