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Sleep-enhancing peptides exert systemic effects by acting on circadian regulators (e.g., CLOCK, BMAL1), restoring pineal-mitochondrial signaling, and enhancing endogenous GH release (Table 3)

Thireou, T

To measure iron concentration in whole-cell lysates, pelleted cells were lysed in optimized 2 RIPA lysis buffer (20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 0.2% SDS, 0.1% sodium deoxycholate, 2% Triton X-100, 0.1 M HCl) for 20 min on ice, followed by centrifugation at 10,000 g for 10 min at 4 C

Cell Stress Chaperones

As glutathione promotes these proteins, it smoothens the skin, reduces fine lines, and improves elasticity, resulting in a more youthful appearance