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HPLC analysis confirms the purity of the peptide, mass spectrometry (MS) verifies the molecular identity, and additional ICP or AAS analysis determines the copper content in the complex

Downregulation of PCBP1/2 mediates Fe 2+ release and induces ferroptosis To explore the potential mechanism by which ACE elevates Fe 2+ levels, iron metabolism proteins associated with ACE were screened by Western blot based on proteomic results (Fig