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In pancreatic cell lines, hypoxia downregulated the expression of full-length WRS and upregulated T2-TrpRS (Paley et al., 2011)

The cells were cultured in neurobasal medium (NBM) supplemented with 2% B27, 1% glutamine, epidermal growth factor (EGF, 10 ng/ml), and basic fibroblast growth factor (bFGF, 10 ng/ml)

Another approach that some researchers have used to enhance oral delivery of drugs is to give them along with additional drugs that help them cross through the intestinal tissue

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