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Eur J Surg Oncol (2014) 40(9):114350

b , Proportion of cells expressing the BFP reporter present in the gRNA plasmid at the initial time point (0 weeks) and late time point (3 weeks)

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Between 4 and 18 months (usually around 6-12 months), parents notice: Loss of skills (stops babbling, stops reaching, loses social smile) Increasing irritability and inconsolable crying Sleep problems Loss of muscle tone (baby feels floppy) Seizures may begin This pattern normal development followed by regression with movement problems is highly suggestive of CFD

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