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J., Pankratov Y

Im a healthy skeptic

To examine whether this interaction is cell-cycle-regulated and/or PARP-activity-dependent, we next conducted in situ PLA assays at physiologic protein levels using RSMC-3Flag cells synchronized by a DT block before release into S phase

rabbit anti-caspase3 (ab32351, 1: 1,000), rabbit anti-active caspase 3 (ab2302, 1: 500), rabbit anti-Bcl2 (ab59348, 1: 1,000), rabbit anti-PKC (ab19031, 1: 500), mouse anti-Erk1/2 (ab54230, 1: 1,000), rabbit anti-phospho-Erk1 Tyr202 and Erk2 Tyr185 (ab201015, 1: 1,000), rabbit-anti-Flit1/VEGFR1 (ab2350, 1: 500), rabbit anti-KDR/VEGFR2 (ab39256, 1: 600), rabbit anti-human CD31 (ab28364, 1: 250), rabbit anti-Von Willebrand Factor antibody (ab6994, 1: 250), and rabbit anti-PlGF (ab9542, 1: 500) antibodies from Abcam (Cambridge, MA, USA)