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The endpoint absorbance of each well was measured 6 times at 450 nm by Epoch 2 microplate spectrophotometer (Agilent BioTek, USA)

HZ was responsible for experiment supplementation and manuscript modification

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Comment: Some commenters recommended engaging ASCs to determine the most effective means to collect data on patient understanding of discharge instructions

Focal adhesion and ECM-receptor interaction pathways exhibited sustained upregulation across all phases, highlighting enhanced extracellular matrix remodeling and cell-matrix communication as organoids matured