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If you suspect degradation, do not use the vial

Treatment of BMDMs with spermine did not induce the proteolytic cleavage of caspase-1, GSDMD or GSDME (Supplementary Fig

Joshi SG, Cooper M, Yost A, Paff M, Ercan UK, Fridman G, Brooks AD (2011) Nonthermal dielectric-barrier discharge plasma-induced inactivation involves oxidative DNA damage and membrane lipid peroxidation in Escherichia coli

Derivatives from simple compounds such as 3-Hydroxyflavone (Dai et al., 2017), as well as more intricate copper ionophores like Hydrophilic Temperature-Sensitive Liposomes (Gaal et al., 2020) and a copper ionophore designed using salicylaldehyde isonicotinoyl hydrazone (Ji et al., 2018), also increase copper levels inside cells

The oral treatment helped too, though the effects were not as strong