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Clear reconstituted peptides left out for under 12 hours may still have partial viability, but conservative protocol recommends disposal

Takouli L, Hadjiyannakos D, Metaxaki P, Sideris V, Filiopoulos V, Anogiati A, et al

To prepare the sucrose solution and water, approximately 40 mL of water should be added to a 50 mL centrifuge tube, which is then sealed with a straw cap