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Their effectiveness depends heavily on the environment you create around them

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J Leukoc Biol (2021) 110(1):2126

we have to do what we can to keep as well as possible

Viruses (2 10 11 PFU per mouse) were purified using cesium chloride gradient centrifugation, dialyzed in PBS containing 10% glycerol, and administered via tail vein injection 14 days before euthanasia