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After centrifugation, SNOTRAP labeling stock solutions (in 40% acetonitrile (ACN)) were added to all samples to reach a final concentration of 1.5 mM (in 50 mM HEPES buffer at pH 7.7) to selectively convert SNO to stable disulfide-iminophosphorane

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Table 129: on the glucose of 5.5mM and 22mM, under normal oxygen and hypoxia condition, the ECAR value of PaCa-2 cell under CoQ10 existence or non-existent situation Table 129 has been described the ECAR value of PaCa-2 cell.Opposite with normal cell, cancerous cell tends to use high glucose to carry out ATP on phenotype and produces (enhanced glycolysis), thereby causes 21mpH/ minute higher ECAR (table 129, the ECAR of untreated normal oxygen 17mM).Utilize the processing of CoQ10 under these conditions, to produce the remarkable minimizing that ECAR leads, most probable ground is related with the minimizing of the lactic acid that glycolysis produces.The relevant minimizing of OCR maybe be relevant with the effect of the mitochondrion oxidative phosphorylation enzyme system that increases in these cells

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