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Washed beads were eluted in 20 l 0.2% SDS in 50 mM HEPES with 4 Laemmli sample buffer (Bio-Rad, 1610747) and boiled at 95 C for western blot analysis

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Likewise, the use of exogenous NP or the blockade/activation of NT/NP receptors has shown beneficial effects, attenuating the inflammatory mechanisms and decreasing AHR (Krishnakumar et al., 2002

Spondyloarthritis associated with familial Mediterranean fever: successful treatment with anakinra