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Description

Clarified lysate was next applied to a Ni Sepharose 6 FF column (Cytiva) linked to an AKTA Avant150 FPLC system for immobilized metal affinity chromatography

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10.3390/jpm11080806 61 TanguayP.MarquisN.GabouryI.KairyD.TouchetteM.TousignantM.et al (2021)

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Gently inject the solution into the vial of lyophilized powder, angling the needle so the liquid runs down the inside wall of the vial

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