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Cell pellets were collected and lysed in 9 M urea or RIPA buffer (10 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% Na deoxycholate, 0.1% SDS, and 1% Triton X)

Harada K, Wang X, Shimodaira Y, Sagebiel T, Bhutani MS, Lee JH, Weston B, Elimova E, Lin Q, Amlashi FG, Mizrak Kaya D, Lopez A, Blum Murphy MA, Roth JA, Swisher SG , Skinner HD, Hofstetter WL, Rogers JE, Thomas I, Maru DM, Komaki R, Walsh G, Ajani JA

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5 Sugars (dietary fiber), the gut microbiota, and depression Carbohydrates serve as the primary energy source for all living organisms to sustain life activities