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Improved Mood and Mental Clarity: Vitamin B12 is vital for brain health

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To quench the metabolism, 500 l of precooled -48 C 100% methanol (Sigma, Germany) was added to the cells, which were then incubated for 30 minutes at -80 C

Additionally, the effect of ETS1 on GPX4 promoter activity was tested by co-transfecting cells with pGL3-GPX4-Promoter or pGL3-Basic, and pcDNA3.1-ETS1 or its control