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doi: 10.3390/brainsci12060806 127 PontifexMGMartinsenASalehRNMHardenGTejeraNMllerMet al

& Bardwell, J

however, host responses may be blocked by expressed viral proteins that are soluble and secreted

Additionally, 10 L Triton X-100 1% (v/v) (X100-5ML, Sigma-Aldrich) and 10 L lysis buffer from the p24 ELISA kit (HIV Type 1 p24 Antigen ELISA, Zeptomatrix) to make a total of 100 L sample and store at 20 C O/N until further processing

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