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doi: 10.1016/j.immuni.2023.12.009 8 AweT.FasaweA.SaweC.OgunwareA.JamiuA

Lysates were complemented with 0.6 U/mL glutathione reductase (Sigma, G3664), 0.2 mM nicotinamide adenine dinucleotide phosphate hydrogen (NADPH, Sigma, N7505), 3 mM reduced glutathione (GSH, Sigma, G4251) and 200 M of the substrate cumene hydroperoxide (CHP, Sigma, 247502)

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Elevated levels of sFASL have been observed in patients with MASLD, and this increase is thought to reflect enhanced apoptotic activity in hepatocytes

Niebler M, Qian X, Hfler D, Kogosov V, Kaewprag J, Kaufmann AM, Ly R, Bhmer G, Zawatzky R, Rsl F, Rincon-Orozco B