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Digest the tissue at 20C for 3 h, with shaking at 40 rpm for the first and last 30 min

Interestingly, in the context of the transgenic mice expressing the mutant human B-crystallin, heterozygous knockout of Nrf2 lessened reductive stress decreasing the expression of the enzymes involved in GSH biosynthesis and recycling, resulting in a decrease in GSH concentration and the GSH/GSSG ratio

In contrast, ferroptosis has emerged as a key driver of ARDS pathogenesis, contributing to epithelial and endothelial cell injury, oxidative stress, and immune dysfunction

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They could be successfully used for our blocking-labeling assay (Supplementary Fig