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The cells transduced with either the HOXC8-overexpressing or mock control vector were subjected to drug selection with 10 g/ml hygromycin for one month, resulting in the establishment of stable HOXC8-overexpressing cell lines (HOXC8-imESCs) and corresponding mock control lines (Control-imESCs)

Balduini A, Di Buduo CA, Malara A, et al

Given the characteristics of short-lived free radical species, which are challenging to confirm as transferring between cells through gap junctions after APAP overdose, we employed a thioacetamide-induced hepatotoxicity model

This significance was measured using a subjective rating scale, with participants selecting their pain on a scale ranging from 0 (no pain at all) to 10 (extreme pain)