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The reaction mixture was diluted with DCM and extracted with water

Abbreviations: Ct = threshold cycles, Cre = Cre recombinase-mediated DNA recombination, eWAT = epididymal white adipose tissue, iWAT = inguinal white adipose tissue, MA = mature adipocytes, ND = non-detectable

Active or history of malignancy: GH/IGF-1 stimulation is contraindicated where there is active cancer, per Tesamorelins prescribing information GH signaling can be growth-promoting