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After TMB substrate solution is added, only those wells that contain Human FGF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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Reliable detection of Ca 2+ influx in rice cells To study the role of cytoplasmic Ca 2+ influx in plant cells, it is absolutely necessary to accurately monitor fine spatial and temporal changes in intracellular Ca 2+ concentration ( 2+ -sensitive bioluminescent photoprotein aequorin, made it possible to detect changes in cytoplasmic Ca 2+ in the submicromolar range (Shimomura et al., 1963)