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The centrifugation process was performed after 10 min at 400g for 10 min, followed by separation and disposal of the plasma and buffy coat

Marklund SL, Holme E, et al

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Scicutella F, Mannelli F, Daghio M, et al

For protein quantification, the remaining 50 L of the cell suspension underwent 3 freeze-thaw cycles using liquid nitrogen, followed by centrifugation at 13,800 g for 10 min