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Results scRNA-seq and identification of root tip cell clusters To explore the molecular mechanisms underlying maize root development at the single-cell transcriptome level, we utilized maize inbred line B73 root tips from 7-day-old seedlings to generate protoplasts for single-cell transcriptome analysis using the 10 Genomics platform ( Figure 1A )

In pernicious anemia, for instance, a lack of intrinsic factor necessary for B12 absorption results in malabsorption

The preprocessed three-dimensional matrix table was imported into SIMCA-P software (Umetrics, Umea, Sweden) for multivariate data analysis

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Cell Metab 18: 239250, 2013 [DOI] [PMC free article] [PubMed] [Google Scholar] 43