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If tests are negative or not feasible to perform, but suspicion remains, consider HLA-DQ genotyping to assess genetic susceptibility and to support long term decision making for first degree relatives

Handling & Preparation Reconstitute using bacteriostatic water per laboratory protocol Use only trained professionals Follow institutional research and safety standards Storage Instructions To preserve potency and stability: Store lyophilized vials in a cool, dry environment Refrigerate reconstituted solutions at 28C Protect from heat, light, and moisture Avoid repeated freezethaw cycles Possible Observations in Research Settings Reported observations may include: Mild redness at administration site Temporary dizziness or headache Transient nausea All findings should be documented and evaluated within controlled research environments

Mouse treatment For all experimental procedures, Tbx1 + / and WT mice were injected intraperitoneally with vB12 dissolved in PBS 20 mg/kg (stored away from light) or with PBS, twice a week for 4 wk starting at 4 wk of age

Growing evidence for using PCT as a diagnostic biomarker for early detection of sepsis Currently there is no recognised diagnostic test available for sepsis diagnosis as stated by the Sepsis-3 task force [5]