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Interestingly, DHA-TF adducts exhibited different effects in the tested cell lines, which does not correspond to their TfR cell surface expression level

A delicate balance between ROS and antioxidants (The cellular redox state) is needed for T-cell receptor signalling to launch an effective immune response

The findings highlight pathways such as fibroblast-to-myofibroblast transition (FMT), redox balance regulation, lysosomal dysfunction, ETC dysfunction, autophagy, and calcium dysregulation as potential contributors to hEDS pathogenesis

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Briefly, cells were incubated for 20 min with anti-mouse CD16/32 to block Fc receptors (BD Bioscience) in FACS buffer (PBS, 0.25% BSA, 1 mM NaN 3 ) and were stained for surface markers