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Slides were then air dried, mounted with Prolong Diamond Anti-fade mounting medium (P36965, Life Technologies) and stored in a light-proof box at 4C before imaging

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For IHC, a one-step IHC assay (KeyGen, KGOS60) was used to stain 7 m thick sections, and the paraffin sections were incubated with anti-GPX4 (Affinity, DF6701) and HO-1 (Affinity, AF5393)